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Overview
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IgG Purification Kits are used for isolation and purification of immunoglobulin G of goat, mouse, rabbit, and other animals. The kit contains immobilized protein A or G and buffer solutions for maximum recovery of IgG. The total time to isolate and purify IgG from serum or other solutions containing IgG is about 30 min. Since the support of protein A or G is silica-base gel, the volume of the retained solution on the gel after the centrifugation is very small. Therefore, all proteins or other materials that do not bind to protein A can be removed by washing twice. Additionally, denaturing of IgG during the elution process is minimal because IgGs bound to the gel are released with a very quick process. The gel in this kit can be used repeatedly 20 times or more with an equal performance. Used gel is also stable for one year in Washing buffer at 0-5°C .
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- Properties
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Overview