Peroxidase Labeling Kit-NH2 - Amerigo Scientific

- Overview
  - Peroxidase Labeling Kit-NH2 is used mainly for the preparation of peroxidase-labeled IgG for enzyme immunoassay (EIA) and for the preparation of peroxidase-labeled antigen for competitive EIA. NH2-reactive peroxidase, a component of this kit, has succinimidyl groups (NHS) and reacts with proteins or other molecules that have an amino group in their structures. This kit contains all the reagents necessary for the labeling process, including storage buffer. The labeling process is simple: mix IgG with NH2-reactive peroxidase and incubate at 37°C for 2 hours. The NH2-reactive peroxidase forms a covalent link with the target molecule without any activation process. The distance of the NHS from peroxidase is about 1.2 nm, half of the radius of the peroxidase molecule. Therefore, when the peroxidase-labeled IgG is used for EIA, the labeling efficiency of the NH2-reactive peroxidase is high enough to eliminate the purification process after labeling. Also, peroxidase labeling will not affect the affinity of the target molecule. If a high-purity conjugate is required after labeling, simply use an affinity column or a gel-permeation column. When labeling small molecules, excess molecules can be removed by using the filtration tubes included in this kit. Because the amino groups of NH2-reactive peroxidase are blocked, no self-conjugation is possible.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Enzyme labeling
    
    Storage
    
    0-5°C
    
    Shipping
    
    with blue ice
    
    * For research use only
- Applications
  - Application
    
    Western blot; Microscopy; Microplate assay
- Reference
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