HiLyte Fluor 647 Labeling Kit-NH2

HiLyte Fluor* 647 Labeling Kit-NH2 is mainly used for the preparation of red fluorescence-labeled proteins, such as IgG, for immunostaining, and cellular proteins for tracing. NH2-reactive HiLyteFluor 647, a component of this kit, has a succinimidyl group (NHS) that reacts with amino groups on proteins or other molecules. This kit contains all the reagents necessary for labeling. Each tube of HiLyte Fluor 647 can label up to 200 μg of IgG, conjugating about 4 to 6 HiLyte Fluor 647 molecules per IgG molecule. The labeling process is simple-add the NH2-reactive HiLyte Fluor 647 to IgG solution on a membrane and incubate at 37°C for 10 minutes. The excess HiLyte Fluor 647 molecules can be removed by a filtration tube. The excitation and emission wavelengths of the HiLyte Fluor 647-labeled IgG are 652 nm and 673 nm, respectively. *HiLyte Fluor is a trademark of AnaSpec, Inc.

Please contact us at for specific academic pricing.