Nitrocefin

- Overview
  - Nitrocefin is a chromogenic cephalosporin used to detect β-lactamases produced by β-lactam resistant bacteria. It does not appear to have antimicrobial properties, but has useful diagnostic properties. It can be used as a reagent in β-lactamase activity studies. Nitrocefin is soluble in DMSO.
    This product is considered a dangerous good. Quantities above 1 g may be subject to additional shipping fees. Please contact us for details.
    
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    Background
    
    β-lactamases hydrolyze the amide bond between the carbonyl carbon and the nitrogen in the β-lactam ring of Nitrocefin. Intact Nitrocefin is converted to hydrolyzed Nitrocefin under visible light (~ 380-500 nm) and a color change occurs from yellow (max at pH 7.0 = 390 nm) to red (max at pH 7.0 = 486 nm).
- Properties
  - CAS Number
    
    41906-86-9
    
    Molecular Formula
    
    C21H16N4O8S2
    
    Molecular Weight
    
    516.51
    
    Appearance
    
    Orange or yellow powder
    
    Solubility
    
    Soluble in DMSO
    
    Other Properties
    
    Source: Synthetic
    Purity Level: (HPLC): ≥95.0%
    
    Storage
    
    -20°C; Protect from light; Store with inert gas.
    
    * For research use only
- Applications
  - Application Description
    
    Microbiology Applications: Nitrocefin is used to detect β-lactamase activity from suspected β-lactam resistant bacteria (see Protocol). It is commonly used at a working concentration of 0.5 - 1.0 mg/ml.