Inactive human tissue transglutaminase

- Overview
  - His6-rhTG2-Cys277Ser is based on the TGM2-allele from I.M.A.G.E.-clone IMAGp958L121020 isolated from neuroblastoma cells of the human brain (Val224-allele, Kanchan et al., Biochem. J. 2013, 455:261–72). It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MAHHHHHHAEELV. At position 277 Cysteine has been replaced by Serin. Please note that numerotation corresponds to the wild type protein without hexahistidine-tag. His6-rhTG2 is produced in E. coli and purified by ion metal chelating chromatography to more than 95 % purity.
    
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    More Details
    
    Zedira
- Properties
  - Name
    
    Tissue-type Transglutaminase, TG2, TGase 2, proteinglutamine-γ-glutamyltransferase
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    > 95 % (visually by SDS-PAGE)
    
    Formulation
    
    The Transglutaminase is lyophilized from 50 mM NaH2PO4, 150 mM NaCl, pH 8.
    
    Storage
    
    Store at ≤ - 20°C. Store working aliquots at ≤ - 20°C. Avoid repeated freezing and thawing. Delivery is possible at ambient temperature
    
    Note
    
    INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
    
    Class
    
    Human Transglutaminase 2
    
    Reconstitution
    
    Add the volume of water specified in the certificate of analysis under aliquotation to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots. For short term storage keep cooled on ice.
- Applications
  - Application Description
    
    His6-rhTG2-Cys277Ser may be used for immunoprecipitation.
- Reference
  - Shinde et al., J. Mol. Cell Cardiol. 2018, 117:36-48
    Van den Akker et al., PLoS ONE 2011, 6:e23067