Human tissue transglutaminase

Catalog Number:
E001055252ZED
Mfr. No.:
T022
Price:
$748
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      • Overview
        • Please contact us at  for specific academic pricing.

      • Properties
        • Source
          Insect cells
          Type
          Recombinant Proteins
          Purification
          > 90 % (visually by SDS-PAGE)
          Formulation
          The Transglutaminase is lyophilized from 10 mM Tris-HCl pH 8.1, 150 mM NaCl, 1 mM EDTA, 5 mM DTT. Sample contains maltodextrin.
          Storage
          Store working aliquots at ≤ - 20°C. Avoid repeated freezing and thawing. Delivery is possible at ambient temperature.
          Note
          INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
          Molecular Weight
          78 kDa (Val224-allele, Kanchan et al., Biochem. J. 2013, 455:261–72)
          Class
          Human Transglutaminase 2
          Reconstitution
          Add the volume of water specified in the certificate of analysis under aliquotation to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots.
          Activity
          His6-rhTG2 is a Ca2+-dependent enzyme. Add 10 mM Ca2+ to activate His6-rhTG2.
      • Applications
        • Application Description
          His6-rhTG2 catalyzes acyl transfer reactions from glutamin residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rhTG2 may also be used for immunoprecipitation.
      • Reference
        • Engstrom et al., Sci. Rep. 2017, 7:77
          Hardes et al., Anal. Biochem. 2012, 428:73-80

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