Human tissue transglutaminase

- Overview
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    Zedira
- Properties
  - Name
    
    Tissue-type Transglutaminase, TG2, TGase 2, proteinglutamine-γ-glutamyltransferase
    
    Source
    
    Insect cells
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    > 90 % (visually by SDS-PAGE)
    
    Formulation
    
    The Transglutaminase is lyophilized from 10 mM Tris-HCl pH 8.1, 150 mM NaCl, 1 mM EDTA, 5 mM DTT. Sample contains maltodextrin.
    
    Storage
    
    Store working aliquots at ≤ - 20°C. Avoid repeated freezing and thawing. Delivery is possible at ambient temperature.
    
    Note
    
    INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
    
    Molecular Weight
    
    78 kDa (Val224-allele, Kanchan et al., Biochem. J. 2013, 455:261–72)
    
    Class
    
    Human Transglutaminase 2
    
    Reconstitution
    
    Add the volume of water specified in the certificate of analysis under aliquotation to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots.
    
    Activity
    
    His6-rhTG2 is a Ca2+-dependent enzyme. Add 10 mM Ca2+ to activate His6-rhTG2.
- Applications
  - Application Description
    
    His6-rhTG2 catalyzes acyl transfer reactions from glutamin residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rhTG2 may also be used for immunoprecipitation.
- Reference
  - Engstrom et al., Sci. Rep. 2017, 7:77
    Hardes et al., Anal. Biochem. 2012, 428:73-80

Datasheet

MSDS

Human tissue transglutaminase

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