Human tissue transglutaminase

- Overview
  - His6-rhTG2 is based on the TGM2-allele from I.M.A.G.E.-clone IMAGp958L121020 isolated from neuroblastoma cells of the human brain (Val224-allele, Kanchan et al., Biochem. J. 2013, 455:261–72). It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MAHHHHHHAEELV….His6-rhTG2 is produced in E. coli and purified by ion metal chelating chromatography to more than 90% purity.
    
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    More Details
    
    Zedira
- Properties
  - Name
    
    Tissue-type Transglutaminase, TG2, TGase 2, proteinglutamine-γ-glutamyltransferase
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    > 90 % (visually by SDS-PAGE)
    
    Formulation
    
    The Transglutaminase is lyophilized from 50 mM NaH2PO4, 150 mM NaCl, pH 8 and less than 0.1 mM Imidazole. Sample contains maltodextrin.
    
    Storage
    
    Store at ≤ - 20°C. Store working aliquots at ≤ - 20°C. Avoid repeated freezing and thawing. Delivery is possible at ambient temperature.
    
    Note
    
    INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
    
    Molecular Weight
    
    78 kDa
    
    Class
    
    Human Transglutaminase 2
    
    Reconstitution
    
    Add the volume of water specified in the certificate of analysis under aliquotation to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots.
    
    Activity
    
    The Transglutaminase is activated with 10 mM Ca2+; due to the precipitation of Calcium Phosphate a buffer exchange (e. g. Tris-Buffer) prior to activation is highly recommended.
- Applications
  - Application Description
    
    His6-rhTG2 catalyzes acyl transfer reactions from glutamin residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rhTG2 may also be used for immunoprecipitation.
- Reference
  - Chrobok et al., PLoS One. 2018, 13:e0196433
    Hietikko et al., Acta Derm Venereol. 2018, 98:366-72
    Sánchez-Lara et al., Vet Pathol. 2015, 52:513-23
    de Jager et al., J. Neurochem. 2015, 34:1116-28
    Fukui et al., FEBS J. 2013, 280:1420-9
    Van den Akker et al., PLoS ONE 2011, 6:e23067
    Schaertl et al., J. Biomol. Screen. 2010, 15:478-87
    Byrne et al., Clin. Immunol. 2010, 136:426-31
    Yamane et al., FEBS J. 2010, 277:3564-74
    Perez Alea et al., Anal. Biochem. 2009, 389:150-6