Human tissue transglutaminase

Catalog Number:
E001055253ZED
Mfr. No.:
T034
Price:
$748
  • Size:
    100 µg
    Quantity:
    Add to Cart:
      • Overview
        • Please contact us at  for specific academic pricing.

          Background

          Transglutaminases are a family of enzymes that catalyze the posttranslational modification of proteins by inserting an isopeptide bond within or between polypeptide chains. These enzymes catalyze the acyl transfer reaction between the γ-carboxyamide groups of peptide-bound glutamine residues and a variety of primary amines, particularly the ε-amino group of lysine. The resulting crosslink is of great significance, since it is highly stable and also resistant to mechanical and proteolytic degradation.

      • Properties
        • Source
          HEK293F cells
          Type
          Recombinant Proteins
          Purification
          > 95 % (visually by SDS-PAGE)
          Formulation
          The Transglutaminase is lyophilized from 10 mM Tris-HCl pH 7.4, 300 mM NaCl, 1 mM EDTA, 1 mM DTT. Sample contains maltodextrin.
          Storage
          Store working aliquots at ≤ - 20°C. Avoid repeated freezing and thawing. Delivery is possible at ambient temperature
          Note
          Intended for research use only, not for use in human, therapeutic or diagnostic applications.
          Molecular Weight
          78 kDa (Val224-allele, Kanchan et al., Biochem. J. 2013, 455:261–72)
          Class
          Human Transglutaminase 2
          Reconstitution
          Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots.
          Activity
          His6-rhTG2 is a Ca2+-dependent enzyme. Add 10 mM Ca2+ to activate His6-rhTG2.
      • Applications
        • Application Description
          Labelling, immobilisation, conjugation and modification of proteins.
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