Transglutaminase Assay Kit, fluorescent

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    1 Kit
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      • Overview
        • Transglutaminases are able to also cleave the isopeptide bond. This principle is used to provide easy to handle, robust and precise fluorogenic assays suitable for screening of compound libraries and drug development. Further, it reliably detects levels as low as 5% of the physiological amount of FXIIIa (0.05 IU/ml) in plasma (Oertel et al., Anal. Biochem. 2007, 367:152-8).
          Mechanism: transglutaminase cleaves the isopeptide bond releasing the dark quencher (2,4-dinitrophenyl) linked to the cadaverine spacer. Subsequently, the increase of fluorescence results from the N-terminally attached fluorophore 2-aminobenzoyl (2-Abz).

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      • Properties
        • Formulation
          (1) SUBSTRATE REAGENT: 2 x 0,185 mg monodansylcadaverine
          (2) BUFFER REAGENT: 2 x 20 ml lyophilized TRIS buffer pH 8,0 containing calcium chloride, sodium chloride, N,N-dimethylcasein, glutathione.

          * Intended for research use only, not for use in human, therapeutic or diagnostic applications.

      • Applications
        • Application
          Transglutaminase Fluorogenic Assay
      • Reference
        • Lorand et al., Anal. Biochem. 1971, 44:221-31

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