Human epidermal transglutaminase, Dispase activated

- Overview
  - Active His6-rhTG3 is based on the TGM3-allele from clone DKFZp686J0716 (isolated from different tissues), corrected by the insertion of the missing T at Position 435. It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MHHHHHHAALGV… .His6-rhTG3 is produced in insect cells and purified by ion metal chelating and ion exchange chromatography to more than 95 % purity. The active Form is obtained by treatment with Dispase I. Active His6-rhTG3 is a Ca2+-dependent enzyme.
    
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    Zedira
- Properties
  - Name
    
    TG3a, TGase 3a, active epidermal protein-glutamin-ε-γ-glutamyltransferase
    
    Source
    
    Insect cells
    
    Type
    
    Recombinant Proteins
    
    Formulation
    
    The Transglutaminase is lyophilized from 20 mM MOPS pH 6.0, 1 mM EDTA and 1 mM DTT. Sample contains maltodextrin.
    
    Storage
    
    Storage for several months is possible at - 20°C.
    
    Note
    
    INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
    
    Molecular Weight
    
    78 kDa (52kDa + 25kDa)
    
    Class
    
    Transglutaminase 3
    
    Reconstitution
    
    Add the desired volume H2O to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be cooled on ice for short term storage. Storage for several days is not recommended.
- Applications
  - Application Description
    
    Active His6-rhTG3 catalyzes acyl-transfer reactions from glutamine residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl)-lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue.Active His6-rhTG3 may also be used for immunoprecipitation and the detection of TG3 autoantibodies.
- Reference
  - Akbar et al., J. Med. Chem. 2017, 60:7910-27

Datasheet

MSDS

Human epidermal transglutaminase, Dispase activated

Human epidermal transglutaminase, Dispase activated

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