Human blood coagulation Factor XIII B subunit

Catalog Number:

E001055245ZED

Mfr. No.:

T050

Price:

$807

Size:

200 µg

Quantity:

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- Overview
  - Recombinant human Factor XIII B subunit is a carrier protein for blood coagulation Factor XIII A subunit. Factor XIII B subunit is a typical mosaic protein and consists of 10 tandem repeats, called “sushi”-domains. Its function is to protect Factor XIII A subunit from slow spontaneous activation and clearance.
    
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    More Details
    
    Zedira
- Properties
  - Name
    
    Recombinant Fibrin stabilizing factor B subunit
    
    Source
    
    Insect cells
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    > 95% [by SDS-PAGE]
    
    Formulation
    
    The recombinant human Factor XIII B subunit is lyophilized from 20 mM Tris-HCl pH 6.8, 150 mM NaCl, 1 mM EDTA.
    
    Storage
    
    Storage for several months is possible at > - 20°C. Delivery is possible at ambient temperature
    
    Note
    
    INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
    
    Molecular Weight
    
    80 kDa (monomer)
    
    Class
    
    Human Factor XIII-B2
    
    Reconstitution
    
    Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots. Keep cooled on ice for short term storage.
- Applications
  - Application Description
    
    Factor XIII B subunit is a carrier protein. The recombinantly expressed protein is able to form a heterotetrameric complex with recombinant Factor XIII A from human, as well as the analogous proteins from pig, dog, mouse, and rat as shown by native PAGE below (Heil et al., 2013). Accordingly, the protein may be used for biochemical studies understanding the physiological function of FXIII, for example determination of the binding constant between Factor XIII A and B subunit (Katona et al., 2014).
- Reference
  - Böhm et al., J. Med. Chem. 2014, 57:10355-65
    Heil et al., Thromb. Res., 2013, 131, e214–e22
    Katona et al., Blood, 2014, 123, 1757-63