CFSE - Amerigo Scientific

- Overview
  - CFSE is cell-membrane permeable and readily accumulates inside viable cells where it covalently attaches to intracellular proteins. Hydrolyzed CFSE emits fluorescence and covalently attached fluorescein molecules do not leak from cells. CFSE-labeled cells can be monitored over several weeks in vivo. Therefore, CFSE is utilized for detection of viable cell as well as for the long-term observation of cell activities by fluorescent microscopy. The excitation and emission wavelengths of CFSE-labeled cells are 500 nm and 520 nm, respectively.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Cell Staining
    
    CAS Number
    
    150347-59-4
    
    Molecular Formula
    
    C29H19NO11
    
    Molecular Weight
    
    557.46
    
    Appearance
    
    white or slightly yellow solid
    
    Storage
    
    -20°C
    
    Shipping
    
    ambient temperature
    
    * For research use only
- Applications
  - Application Description
    
    Staining ProcedurePrepare 1 mM CFSE solution with DMSO. Dilute it to prepare 10-50 μM CFSE solution with PBS or an appropriate buffer. Add CFSE solution with 1/10 of the volume of cell culture medium to the cell culture. Incubate the cell at 37°C for 15 to 30 min. Wash cells twice with PBS or an appropriate buffer. Observe the cells under a fluorescence microscope with 490 nm excitation and 530 nm emission filters.
    
    When it is difficult to take out all the powder from the container,
    please add the solvent into a container and dissolve it before its use.
- Reference
  - 1) M. Bronner-Fraser, Alterations in Neural Crest Migration by a Monoclonal Antibody That Affects Cell Adhesion. J Cell Biol. 1985;101:610-617.
    
    2) A. Nose, et al., A Novel Cadherin Cell Adhesion Molecule:Its Expression Patterns Associated WithImplantation and Organogenesis of Mouse Embryos. J Cell Biol. 1986;103:2649-2658.
    
    3) S. A. Weston, et al., New Fluorescent Dyes for Lymphocyte Migration Studies Analysis by Flow Cytometry and Fluorescent Microscopy. J Immunol Methods. 1990;133:87-97.
    
    4) C. K. Raymond, et al., Molecular Analysis of the Yeast VPS3 Gene and the Role of Its Product in Vacuolar Protein Sorting and Vacuolar Segregation during the Cell Cycle. J Cell Biol. 1990;111:877-892.
    
    5) G. Radcliff, et al., Quantification of Effector/Target Conjugation Involving Natural Killer(NK) or Lymphokine Activated Killer(LAK) Cells by Two-color Flow Cytometry. J Immunol Methods. 1991;139:281-292.
    
    6) S. A. Weston, et al., Calcein: a Novel Marker for Lymphocytes Which Enter Lymph Nodes. Cytometry. 1992;13:739-749.
    
    7) L. S. D. Clerck, et al., Use of Fluorescent Dyes in the Determination of Adherence of Human Leucocytes to Endothelial Cells and the Effects of Fluorochromes on Cellular Function. J Immunol Methods. 1994;172:115-124.

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