Name: Ampicillin Sodium
Price: 141.00 USD

- Overview
  - Ampicillin Sodium is a member of the β-lactam family structurally similar to penicillin. The compound inhibits bacterial cell wall synthesis. Ampicillin resistance is used as a selectable marker to confirm successful cell transformation, as only cells containing plasmid-encoded ESBLs (Extended Spectrum B-lactamases) survive. Ampicillin Sodium is soluble in water.
    
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    Background
    
    Like all β-lactams, Ampicillin interferes with PBP (penicillin binding protein) activity otherwise involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised ultimately leading to cell lysis.
- Properties
  - CAS Number
    
    69-52-3
    
    Molecular Formula
    
    C16H18N3NaO4S
    
    Molecular Weight
    
    371.39
    
    Appearance
    
    White or almost white powder, hygroscopic
    
    Solubility
    
    Soluble in water (50 mg/ml)
    
    Other Properties
    
    Source: Semi-synthetic
    Water Content (Karl Fischer): ≤2.0%
    PH: 8.0-10.0
    Assay: (anhydrous basis): 845-988 µg/mg
    Identification: Passes test
    
    Storage
    
    2-8°C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Ampicillin Sodium targets Gram-negative non ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus species and medically important enteric pathogens such as Shigella and Salmonella. Interestingly, ampicillin has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug."
    
    Microbiology Applications: Ampicillin Sodium is often used to select for cells that have been transformed with a plasmid containing the bla gene which confers resistance to Ampicillin. Ampicillin Sodium is typically used at a concentration of 50-100 µg/mL.
    Media Supplements
    Ampicillin Sodium can be used as a selective agent in several types of isolation media:
    Aeromonas Medium Base - Ampicillin Selective Supplement
    
    Eukaryotic Cell Culture Applications: Ampicillin is routinely used to select for cells containing the pcDNA3.1 and pEAK10 resistance plasmids in cell line A904L at an effective concentration of 50 µg/mL. For additional information on your cell culture needs, please visit our cell-culture database.