Name: Ampicillin ReadyMade™ Solution
Price: 177.00 USD

- Overview
  - Ampicillin ReadyMadeTM solution is provided as a sterile-filtered solution of Ampicillin Anhydrous formulated in DMSO at a concentration of 100 mg/ml. It has been filter-sterilized using a 0.2 μm filter.
    Ampicillin Anhydrous is a member of the β-lactam family similar in structure to penicillin.
    
    Please contact us at for specific academic pricing.
    
    Background
    
    Like all β-lactams, Ampicillin interferes with PBP (penicillin binding protein) activity otherwise involved in the final phase of peptidoglycan synthesis. PBPs are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised ultimately leading to cell lysis.
- Properties
  - CAS Number
    
    69-53-4
    
    Molecular Formula
    
    C16H19N3O4S (Ampicillin Anhydrous)
    
    Molecular Weight
    
    349.40
    
    Other Properties
    
    Source: Semi-synthetic
    
    Storage
    
    -20°C ; Protect from light
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Ampicillin is broad-spectrum, effective against both Gram-positive and Gram-negative bacteria including Gram-negative non-ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus species and medically important enteric pathogens such as Shigella and Salmonella. Interestingly, Ampicillin has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug." Resistance to Ampicillin is routinely used as a selectable marker to confirm successful cell transformation.
    
    Microbiology Applications: Ampicillin Anhydrous can be used as a selective agent in several types of isolation media:
    Aeromonas Medium Base - Ampicillin Selective SupplementLuria-Bertani (LB) Medium
    
    Eukaryotic Cell Culture Applications: Ampicillin is routinely used to select for cells containing the pcDNA3.1 and pEAK10 resistance plasmids in cell line A904L at an effective concentration of 50 µg/mL. For additional information, please visit our cell-culture database.