Ampicillin Anhydrous

- Overview
  - Ampicillin Anhydrous is a member of the β-lactam family and is similar in structure to penicillin. Ampicillin Anhydrous is the most stable and pure form that we offer. It is sparingly soluble in water and soluble in 1 N NH4OH.
    
    Please contact us at for specific academic pricing.
    
    Background
    
    Like all β-lactams, Ampicillin interferes with PBP (penicillin binding protein) activity otherwise involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised ultimately leading to cell lysis.
- Properties
  - CAS Number
    
    69-53-4
    
    Molecular Formula
    
    C16H19N3O4S
    
    Molecular Weight
    
    349.40
    
    Appearance
    
    White crystalline powder
    
    Solubility
    
    Sparingly soluble in water. Soluble in 1 N NH4OH (50 mg/mL)
    
    Other Properties
    
    Source: Semi-synthetic
    Water Content (Karl Fischer): ≤1.0%
    PH: 3.5 - 5.5
    Assay: (On Dried Basis): ≥98.0%
    Heavy Metals: ≤20ppm
    Optical Rotation: +280° to +305°
    Identification: Passes test
    
    Storage
    
    2-8°C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Ampicillin targets Gram-negative, non ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus and medically important enteric pathogens such as Shigella and Salmonella. Interestingly, Ampicillin has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug."
    
    Microbiology Applications: Ampicillin Anhydrous is often used to select for cells that have been transformed with a plasmid containing the ampR gene which confers resistance to Ampicillin. Ampicillin is typically used at a concentration of 50-100 µg/mL. Media Supplements Ampicillin Anhydrous can be used as a selective agent in several types of isolation media: Aeromonas Medium Base - Ampicililn Selective Supplement
    
    Eukaryotic Cell Culture Applications: Ampicillin is routinely used to select for cells containing the pcDNA3.1 and pEAK10 resistance plasmids in cell line A904L at an effective concentration of 50 µg/mL. For additional information on your cell culture needs, please visit our cell-culture database.