Ampicillin/Sulbactam (2:1)

- Overview
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    Background
    
    Like all β-lactams, Ampicillin interferes with PBP (penicillin binding protein) activity otherwise involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised ultimately leading to cell lysis.
- Properties
  - CAS Number
    
    117060-71-6
    
    Molecular Formula
    
    Ampicillin: C16H19N3O4S
    Sulbactam: C8H11NO5S
    
    Molecular Weight
    
    Mixture
    
    Appearance
    
    White or almost white crystalline powder
    
    Other Properties
    
    PH: 8.0-10.0
    Assay: Ampicillin: ≥563 μg/mg
    
    Storage
    
    <30°C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Ampicillin targets Gram-negative non ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus and medically important enteric pathogens such as Shigella and Salmonella. Ampicillin has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug."
    
    Microbiology Applications: Ampicillin/Sulbactam is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. Media Supplements Ampicillin can be used as a selective agent in several types of isolation media: Aeromonas Medium Base - Ampicillin Selective Supplement
    
    Eukaryotic Cell Culture Applications: Ampicillin is routinely used to select for cells containing the pcDNA3.1 and pEAK10 resistance plasmids in cell line A904L at an effective concentration of 50 µg/mL. For additional information, please visit our cell-culture database.