Rat tissue transglutaminase

- Overview
  - His6-rrTG2 is based on clone IRBPp993H102D. It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MHHHHHHAEELNL.... His6-rrTG2 is produced in E. coli and purified by ion metal chelating chromatography to more than 95 % purity.
    
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    More Details
    
    Zedira
- Properties
  - Name
    
    Tissue-type Transglutaminase, TG2, TGase 2, protein-glutamine-γ-glutamyltransferase
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    > 95 % (visually by SDS-PAGE)
    
    Formulation
    
    The Transglutaminase is lyophilized from 50 mM NaH2PO4, 150 mM NaCl, pH 8. Sample contains maltodextrin.
    
    Storage
    
    Store working aliquots at ≤ - 20°C. Avoid repeated freezing and thawing. Delivery is possible at ambient temperature
    
    Note
    
    INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
    
    Molecular Weight
    
    77 kDa
    
    Class
    
    Animal Transglutaminase 2
    
    Reconstitution
    
    Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots. Keep cooled on ice for short term storage.
    
    Activity
    
    Add 10 mM Ca2+ to activate His6-rrTG2.
- Applications
  - Application Description
    
    His6-rrTG2 catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rrTG2 may also be used for immunoprecipitation.
- Reference
  - Sánchez-Lara et al., Vet Pathol. 2015, 52:513-23