pAAVone-sc-AAV13-CMV-EGFP

pAAVone-sc-AAV13-CMV-EGFP is a plasmid used to produce scAAV13-CMV-EGFP using novel AAVone production system. It integrates all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and AAV13), and a self-complementary AAV-CMV-EGFP genome. scAAV13-CMV-EGFP viruses can be simply and efficiently produced by transfection of this single-into host cells, such us HEK 293 and HEK 293T. AAV13 is a relatively lesser-known and less commonly used serotype of adeno-associated virus. It is part of the AAV clade with similarities to other serotypes such as AAV1 and AAV7. AAV13 has unique tissue tropism, but it has not been as extensively studied or utilized in gene therapy compared to more common serotypes like AAV2, AAV9, or AAV8.

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