Nourseothricin (Streptothricin) Sulfate

- Overview
  - Nourseothricin (Streptothricin) Sulfate is a broad-spectrum Streptothricin antibiotic and peptidyl nucleoside derived from Streptomyces noursei. Structurally, itis a streptolidine ring bound through carbon 1 of a 4-o-acetyl,N-poly-lysinylated glucosamine. It is used as a selection agent for genetically modified bacteria, yeast, plants, fungi, and protozoa. The polycationic antibiotic is a mixture of several streptothricins, mainly D and F.
    Nourseothricin (Streptothricin) Sulfate is soluble in water.
    
    Please contact us at for specific academic pricing.
    
    Background
    
    Nourseothricin Sulfate inhibits protein synthesis by inducing miscoding of mRNA. It interferes with the outer membrane of Gram-negative bacteria by passing through the outer membrane very slowly via the porin pores.
- Properties
  - CAS Number
    
    96736-11-7
    
    Molecular Formula
    
    C50H92N20O18 • H2SO4
    
    Molecular Weight
    
    1359.46
    
    Solubility
    
    Soluble in water
    
    Storage
    
    2-8 °C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Nourseothricin is a broad-spectrum activity against Gram-positive and Gram-negative bacteria. It is also active against viruses, yeast (including C. albicans), viruses, fungi, mycobacteria, mycoplasma, and parasites.
    
    Microbiology Applications: Nourseothricin Sulfate is often used to select for cells with a mutation in the nat1 gene which encodes the enzyme nourseothricin acetyltransferase which confers resistance to Nourseothricin. It can be used in yeasts, bacteria, protozoa, plant cells, and filamentous fungi.
    Resistant strains with a streptothricin-inactivating acetyl transferase have an additional protection system by way of a reduced penetrability of the outer membrane. Researchers showed thaat strains could be rendered sensitive by damaging the barrier function of the membrane.