Dihydrostreptomycin Sulfate

- Overview
  - Dihydrostreptomycin Sulfate, a derivative of streptomycin, is a semi-synthetic aminoglycoside with bactericidal properties. The antibiotic interferes with bacterial protein synthesis and is commonly used to study aminoglycoside uptake. It is freely soluble in aqueous solution.
    
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    Background
    
    Aminoglycosides target the 30S ribosomal subunit (irreversible binding to S12 protein) resulting in an inability to read mRNA since this interferes with the initiation complex between the mRNA and the bacterial ribosome. This leads to the synthesis of faulty proteins which results in death of the bacterial cell.
    
    Dihydrostreptomycin inhibited the induction of beta-galactosidase in E. coli. It also caused accumulation of pyruvate, similar to known inhibitors of terminal respiration (Bragg and Polglase 1963).
- Properties
  - CAS Number
    
    5490-27-7
    
    Molecular Formula
    
    (C21H41N7O12)2 •3 H2SO4
    
    Molecular Weight
    
    1461.42
    
    Appearance
    
    White or almost white powder
    
    Solubility
    
    Freely soluble in aqueous solution
    
    Other Properties
    
    Source: Semi-synthetic
    PH: 4.5-7.0
    Assay: (On Dried Basis): ≥730 u/mg
    Loss on Drying: ≤5.0%
    
    Storage
    
    2-8°C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Activity against most Gram-positive and Gram-negative organisms, and Mycobacterium.
    
    Microbiology Applications: Media Supplements Dihydrostreptomycin can be used as a selective agent in several types of isolation media: STAA Agar - STAA Selective Supplement STAA Agar - STA Selective Supplement
    
    Eukaryotic Cell Culture Applications: Research on the use of SPLVs (stable plurilamellar vesicles) to entrap aminoglycosides as a drug carrier. Murine peritoneal macrophases and guinea pig peritoneal macrophages were used to study infections caused by Brucella species (Brucella canis and Brucella abortus) with effective elimination of intracellular bacteria (Fountain et al, 1985). Mouse fibroblasts (LM-strain (CCL1.2)) were used in a sensitive, reliable cell culture assay to determine the water quality of oil-refinery effluents, and Dihydrostreptomycin Sulfate was incorporated in the media to prevent contamination (Richardson, 1977).