Name: HiPure Insect DNA Kit
Price: 275.00 USD

- Overview
  - HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
    
    Principle
    This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
    
    Advantages
    • High purity DNA - can be used in sensitive downstream applications such as multiplex and quantitative pcr
    • Fast - several samples can be extracted in less than 30 minutes
    • Good repeatability - suitable for extracting high-yield DNA from insect tissue samples
    • Safe - without phenol chloroform extraction and alcohol precipitation
    
    Please contact us at for specific academic pricing.
- Properties
  - Details
    
    Purification method: Midi spin column
    Purification technology: Silica technology
    Process method: Manual (centrifugation or vacuum)
    Sample type: Insect tissue samples
    Sample amount: <10 mg
    
    Storage
    
    Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in thiscase buffers should be redissolved before use. Make sure that all buffers areat room temperature when used.
    
    * For Research Use Only
- Applications
  - Application
    
    PCR, southern bolt and virus detection, etc
    
    Application Description
    
    Isolation total DNA from <10 mg insect tissue

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