HiPure Universal RNA Kit

The Kit integrates phenol/guanidine-based lysis and silica membrane purification of total RNA. MagZol Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of samples and inhibit RNases. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable extracting up to 200μg total purified RNA from less than 100mg animal, plant, fungal samples, bacteria, cells and other samples.

Principle
Tissue samples (10-100mg) are homogenized in MagZol Reagent. After addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the spin column, where the total RNA (up to 100µg) binds to the membrane and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30-100µl of RNase-free water.

Advantages
• Efficient DNA removal - one step RNA extraction can effectively remove genomic DNA
• High quality - one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
• Fast - several samples can be extracted in 30 minutes
• High applicability - samples including animals, plants, bacteria, cells, etc.
• High output - enable to process large samples and the yield can be up to 200μg

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