Name: MagPure FFPE DNA/RNA Kit
Price: 448.00 USD

- Overview
  - The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and pyrosequencing.
    
    Principle
    The sample is lysed and digested under the action of lysate and protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, the supernatant contain RNA was collected and bind to MagBind Particles. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Elution Buffer.
    
    Advantages
    • High quality - high purity total RNA / DNA can be directly used in various downstream applications
    • Safe - no phenol chloroform extraction required
    • Simultaneous extraction - simultaneously isolate DNA and RNA from one sample
    • Post digestion sorting - higher DNA and RNA yields
    • High RNA yield - monodisperse carbonyl adsorption
    
    Please contact us at for specific academic pricing.
- Properties
  - Details
    
    Purification method: Polydisperse silicon based magnetic beads (DNA)
    Monodisperse carbonyl magnetic beads (RNA)
    Purification technology: Magnetic beads technology
    Process method: Manual or automatic
    Sample type: FFPE slice, FFPE puncture sample, embedded tissue
    Sample amount: No more than six 10 µm sections of 150 mm2 surface area or three 20µm sections of 150 mm2 surface area
    
    Storage
    
    Proteinase K and MagBind Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
    
    * For Research Use Only
- Applications
  - Application
    
    RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc.
    
    Application Description
    
    Co-isolation DNA and RNA from FFPE tissue

Documents

R6327 MagPure FFPE DNA RNA Kit

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