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Overview
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This Kit is designed forpurification of high quality circulating DNA (cfDNA) from 1-6ml cell-free bodyfluids (such as plasma, serum). The purified DNA is suitable for direct use indownstream applications such as PCR, real-time PCR, Biochip analysis and NGS.
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.
Advantages
• Economy - less than 50% of the price of Qiagen and other imported products
• Automatic - without labour
• High purity - OD 260 / 280 : 1.7-1.9, OD 260 / 230 :1.5-2.0Please contact us at for specific academic pricing.
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- Properties
- Applications
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Overview