MagPure FFPE DNA/RNA Kit

MagPure FFPE DNA/RNA Kit

Catalog Number:
NAPK357143ANG
Mfr. No.:
IVD3026
Price:
$1,119
  • Size:
    200 preps
    Quantity:
    Add to Cart:
      • Overview
        • The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and Pyrosequencing.

          Principle
          FFPE samples are incubated in an optimized lysis buffer, which results in the release of RNA and precipitation of DNA. After centrifugation, the RNA-containing supernatant and DNA-containing pellet are then processed separately to purify RNA and DNA. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by RNase Free Water.

          Advantages
          • Post digestion sorting - higher DNA and RNA yields
          • Economy – the price is much lower than imported reagents

          Please contact us at for specific academic pricing.

      • Properties
        • Details
          Purification method: Polydisperse magnetic beads
          Purification technology: Magnetic beads technology
          Process method: Manual or automatic
          Sample type: FFPE slice, FFPE puncture sample, embedded tissue
          Sample amount: No more than six 10µm sections of 150 mm2 surface area or three 20µm sections of 150 mm2 surface area.
          Storage
          Proteinase K and MagBind Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

          * For Research Use Only

      • Applications
        • Application
          RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc.
          Application Description
          Co-isolation total RNA and DNA from FFPE tissue

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