-SulfoBiotics- Biotin-HPDP(WS) solution

- Overview
  - Biotin-HPDP(WS) is a novel water-soluble biotin-labeling reagent that is modified from Biotin-HPDP (Product code: B573). Biotin-HPDP is a conventional reagent to introduce a biotin moiety to a sulfhydryl group of protein via reversible disulfide bond. This reagent is useful for affinity-purification of biotinylated proteins by using avidin-coated beads, because the disulfide bond is cleavable by a reducing agent. Thus, Biotin-HPDP has been widely used for biotin switch assay, which is an analytical technique for protein thiol modifications such as s-nitrosylation, s-sulfhydration, and s-palmitoylation. However, preparation of the solution is time-consuming due to the low-solubility in water, even DMSO and DMF. Therefore, Biotin-HPDP(WS) solution was developed to improve the solubility of Biotin-HPDP.
    
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- Properties
  - Appearance
    
    Colorless to slightly yellow liquid
    
    Storage
    
    Store at 0-5°C
    
    Shipping
    
    ambient temperature
    
    * For research use only
- Applications
  - Application
    
    Biotin Labeling
- Reference
  - 1) S. R. Jaffrey and Solomon H. Snyder, "The biotin switch method for the detection of S-nitrosylated proteins", Sci. STKE, 2001, 86, pl1.
    
    2) X. Wang, N. Kettenhofen, S. Shiva, N. Hogg, and M. Gladwin, "Copper dependence of the biotin switch assay: modified assay for measuring cellular and blood nitrosated proteins", Free Radic. Biol. Med., 2008, 44, 1362.
    
    3) M. T. Forrester, M. W. Foster, M. Benhar, and J. S. Stamler, "Detection of protein S-nitrosylation with the biotin switch technique", Free Radic. Biol. Med., 2009, 46(2), 119.
    
    4) M. D. Kornberg, N. Sen, M. R. Hara, K. R. Juluri, J. V. K. Nguyen, A. M. Snowman, L. Law, L. D. Hester, and S. H. Snyder, "GAPDH mediates nitrosylation of nuclear proteins", Nat. Cell Biol., 2010, 12(11), 1094.
    
    5) J. Wan, A. F. Roth, A. O. Bailey, and N. G. Davis, "Palmitoylated proteins: purification and identification", Nat. Protoc., 2007, 1573.
    
    6) A. K. Mustafa, M. M. Gadalla, N. Sen, S. Kim, W. Mu, S. K. Gazi, R. K. Barrow, G. Yang, R. Wang, and S. H. Snyder, "H2S signals through protein S-sulfhydration", Sci. Signal., 2009, 2(96), ra72.