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Overview
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Diethyldithiocarbamate (DETC) is a good spin-trapping reagent for nitric oxide in vivo. However, DETC has not been widely utilized for NO detection in biological samples due to its poor water solubility. DTCS, an analog of DETC, forms a water-soluble iron(II) complex (Fe-DTCS). The Fe-DTCS complex then forms a complex with NO (NO-Fe-DTCS). Dr. Yoshimura successfully obtained two-dimensional ESR images of NO, induced by lipopolysaccharide in mouse peritoneum. DTCS sodium salt (DTCS Na) was used for this experiment because it is less toxic than ammonium salt (sodium salt LD50: 1942 mg/kg; ammonium salt LD50: 765 mg/kg). Since the Fe-DTCS complex is more stable than the other dithiocarbamate complexes in the air or in aqueous solutions, it could be a useful spintrapping reagent for biochemical research. The Fe-DTCS complex should be used immediately after preparation. An excessive amount of DTCS Na (usually 5 equivalents DTCS Na to FeSO4) is required to make a more stable solution. Dithiocarbamates tend to decompose under physiological conditions to form toxic carbon disulfide.
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- Properties
- Applications
- Reference
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Overview