Name: Ceftiofur Hydrochloride
Price: 234.00 USD

- Overview
  - Ceftiofur Hydrochloride is the hydrochloride salt of Ceftiofur, a broad-spectrum, third-generation β-lactamase resistant cephalosporin commonly used in veterinary research applications that was first described in 1987. Its metabolite (desfurolyceftiofur) also has antibiotic activity. Ceftiofur Hydrochloride is insoluble in water.
    
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    Background
    
    Like β-lactams, cephalosporins interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to cephalosporins is commonly due to cells containing plasmid encoded β-lactamases. Like many cephalosporins, ceftiofur is resistant to β-lactamases.
- Properties
  - CAS Number
    
    103980-44-5
    
    Molecular Formula
    
    C19H17N5O7S3 • HCl
    
    Molecular Weight
    
    560.02
    
    Solubility
    
    Insoluble in water
    
    Storage
    
    -20°C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Ceftiofur is a broad-spectrum antibiotic targeting a wide variety of Gram-positive and Gram-negative bacteria including the β -lactamase producing strains.
    
    Microbiology Applications: Ceftiofur is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram- negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. Representative MIC values include:E. coli 0.12 µg/mL – 0.5 µg/mL Salmonella spp. 0.5 µg/mL – 2 µg/mL Ceftiofur is resistant to β-lactamases. Resistant strains of E. coli have been reported.
    
    Eukaryotic Cell Culture Applications: Ceftiofur was tested on equine bone marrow-derived mesenchymal stromal cells and did not affect cell viability (Parker et al, 2011). The influence of Ceftiofur was examined in vitro on bovine embryos during oocyte maturation, oocyte fertilization and embryo culture. When oocytes were exposed during oocyte maturation it did not affect embryo development, either at low (10, 50 µg/ml) or high (100, 200 µg/ml) concentrations. However, if fertilized embryos were exposed to 50 µg/ml, it retarded embryo development. Therefore, longterm exposure to high doses during post-fertilization adversely affects embryo development (Holyoak et al, 2009).