Name: Cefazolin Sodium Salt, USP
Price: 195.00 USD

- Overview
  - Cefazolin Sodium Salt, USP is a first generation cephalosporin. It is a semi-synthetic, broad-spectrum β-lactam with bactericidal activity. It can be used to study penicillin binding proteins, especially PBP2 and PBP4 on the synthesis of bacterial cell walls. Cefazolin Sodium is sparingly soluble in aqueous solution.
    
    Please contact us at for specific academic pricing.
    
    Background
    
    Like β-lactams, cephalosporins interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to cephalosporins is commonly due to cells containing plasmid encoded β-lactamases.
- Properties
  - CAS Number
    
    27164-46-1
    
    Molecular Formula
    
    C14H13N8NaO4S3
    
    Molecular Weight
    
    476.49
    
    Appearance
    
    White or almost white powder
    
    Solubility
    
    Freely soluble in water
    
    Other Properties
    
    Source: Semi-synthetic
    Water Content (Karl Fischer): ≤6.0%
    PH: 4.0-6.0
    Assay: (On Dried Basis): 89.1-110.1%
    Optical Rotation: -10° to -24°
    
    Storage
    
    -20°C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Cefazolin Sodium is effective against Gram-positive and Gram-negative bacteria.
    
    Microbiology Applications: Cefazolin Sodium is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. Representative MIC values include:
    Staphylococcus aureus 0.25 µg/mL -32 µg/mL
    Staphylococcus epidermidis ≤0.06 µg/mL － >128 µg/mL
    Media Supplement
    Cefazolin Sodium is routinely used as a selection agent in isolation media:
    Columbia Blood Agar - Campylobacter selective supplement (Butzler)
    
    Eukaryotic Cell Culture Applications: The reducing power of the cell involves Glucose-6-phosphate dehydrogenase (G6PD). The reprograming of nicotinamide adenine dinucleotide (NAD) homeostasis is reported to be an important step in cancer progression. Rat erythrocyte G6PD enzyme was studied in vitro with Cefazolin and it was found that the enzyme increased upon exposure to Cefazolin (Temel et al, 2018).The toxicity of cephalosporin to rabbit kidney cells in vitro was compared to their known nephrotoxic potential in vivo. Researchers found that when they added a microsomal S9 fraction from rabit kidney was added to the culture, Cephalothin was desacetylated and its toxicity to kidney cells was reduced. Desacetyl‐cephalothin was relatively nontoxic to rabbit renal tissue in vitro (Hottendorf et al, 1987).