Biotinylated mouse tissue transglutaminase

- Overview
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    More Details
    
    Zedira
- Properties
  - Name
    
    Tissue-type Transglutaminase, TG2, TGase 2, proteinglutamine-γ-glutamyltransferase
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    > 95 % (visually by SDS-PAGE)
    
    Conjugate
    
    Biotin
    
    Formulation
    
    The Transglutaminase is formulated in 10 mM Sodium Phosphate pH 8.0, 15 mM NaCl. Sample contains 50% glycerol. His6-rmTG2 is a Ca2+-dependent enzyme.
    
    Storage
    
    Store at -20 °C in working aliquots. Repeated freezing and thawing is not recommended.
    
    Note
    
    INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
    
    Molecular Weight
    
    79 kDa
    
    Class
    
    Animal Transglutaminase 2
    
    Activity
    
    Add 10 mM Ca2+ to activate His6-rmTG2.
- Applications
  - Application Description
    
    His6-rmTG2 catalyzes acyl transfer reactions from glutamin residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rmTG2 may also be used for immunoprecipitation.