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Overview
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Biotin Labeling Kit-SH is mainly used for the preparation of biotin-labeled IgG for enzyme immunoassay (EIA). SH reactive biotin, a component of this kit, reacts with sulfhydryl groups of proteins or other molecules. The kit contains all the reagents necessary for the labeling. The reducing agent included in this kit creates sulfhydryl groups in the IgG molecule. Because the reducing agent is a water-soluble phosphine compound, it can be removed by a filtration tube with a simple process. An average of 5 to 8 biotin molecules conjugate to each IgG molecule. The number of biotin molecules per protein can be determined by HABA assay. Excess biotin molecules can be removed by a filtration tube.
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