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Overview
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Alkaline Phosphatase Labeling Kit-NH2 is mainly used for the preparation of alkaline phosphatase-labeled IgG for enzyme immunoassay (EIA) and for the preparation of alkaline phosphataselabeled antigen for competitive EIA. NH2-reactive ALP, a component of this kit, can react with amino groups of proteins or other molecules. This kit contains all of the necessary reagents for the labeling process including Storage buffer. NH2-reactive ALP forms a covalent link with the target molecule without any activation process. The labeling efficiency of the NH2-reactive ALP is high enough to eliminate any purification process after labeling when the alkaline phosphatase-labeled IgG is used for EIA. If a high purity conjugate is required after labeling, simply use an affinity column or a gel permeation column. When labeling small molecules, excess molecules can be removed by using the Filtration tubes included in this kit.
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- Properties
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Overview