Alkaline Phosphatase Labeling Kit-NH2

Alkaline Phosphatase Labeling Kit-NH2

Catalog Number:
CFA1108117DOJ
Mfr. No.:
LK12-10
Price:
$445
  • Size:
    3 samples
    Quantity:
    Add to Cart:
      • Overview
        • Alkaline Phosphatase Labeling Kit-NH2 is mainly used for the preparation of alkaline phosphatase-labeled IgG for enzyme immunoassay (EIA) and for the preparation of alkaline phosphataselabeled antigen for competitive EIA. NH2-reactive ALP, a component of this kit, can react with amino groups of proteins or other molecules. This kit contains all of the necessary reagents for the labeling process including Storage buffer. NH2-reactive ALP forms a covalent link with the target molecule without any activation process. The labeling efficiency of the NH2-reactive ALP is high enough to eliminate any purification process after labeling when the alkaline phosphatase-labeled IgG is used for EIA. If a high purity conjugate is required after labeling, simply use an affinity column or a gel permeation column. When labeling small molecules, excess molecules can be removed by using the Filtration tubes included in this kit.

          Please contact us at for specific academic pricing.

      • Properties
        • Categories
          Enzyme labeling
          Storage
          0-5°C
          Shipping
          with blue ice

          * For research use only

      • Applications
        • Application
          Western blot; Microscopy; Microplate assay
      • Reference
        • 1) B. Pandey, A.V. Demchenko, K.J. Stine, "Nanoporous gold as a solid support for protein immobilization and development of an electrochemical immunoassay for prostate specific antigen and carcinoembryonic antigen", Microchim Acta., 2012, 179, (1-2), 71.

          2) M. Watanabe, I. Takemasa, N. Kaneko, Y. Yokoyama, E. Matsuo, S. Iwasa, M. Mori, N. Matsuura, M. Monden, and O. Nishimura, "Clinical significance of circulating galectins as colorectal cancer markers", Oncol. Rep.., 2011, 25, (5), 1217.

          3) Y. Matsumae, Y. Takahashi, H. Shiku and T. Matsue, "Quantitative Real‐Time Monitoring of Antibody‐Induced Internalization of Epidermal Growth Factor Receptor on Single Living Mammalian Cells Using Scanning Electrochemical Microscopy", ChemElectroChem., 2018, 5, (20), 3096.

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