Z-DEVD-pNA

- Overview
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    Background
    
    Z-DEVD-pNA is a colorimetric substrate for caspase-3 with increased cell permeability. Sequence is based on PARP poly(ADP-ribose) polymerase which is clevaved by caspase-3 at the amino acid sequence DEVD[1]. Z-DEVD-pNA also recognizes caspases-1, -4 and -7. The N-terminus of Z-DEVD-pNA is blocked by a benzyloxycarbonyl (Z) moiety that could increase cell permeability. The Caspase-3 activity is calculated by sepctrophotometric reading of free pNA (l = 400 nm) following cleavage from the substrate DEVD-pNA. [2] Z-DEVD-pNA can be used to identify and quantify caspase-3 activity in apoptotic cell lysates.
    
    1. Lazebnik, Y.A.,Kaufmann, S.H.,Desnoyers, S., et al. Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE. Nature 371, 346-347 (1994).
    2. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature 376, 37 (1995)
- Properties
  - Alternative Name
    
    8-(2-carboxyethyl)-5-(carboxymethyl)-11-isopropyl-14-((4-nitrophenyl)carbamoyl)-3,6,9,12-tetraoxo-1-phenyl-2-oxa-4,7,10,13-tetraazahexadecan-16-oic acid
    
    Molecular Formula
    
    C32H38N6O14
    
    Molecular Weight
    
    730.68
    
    Appearance
    
    A solid
    
    Purity
    
    98.00%
    
    Solubility
    
    ≥28.35 mg/mL in DMSO; ≥4.31 mg/mL in EtOH with ultrasonic; ≥7.31 mg/mL in H2O
    
    Storage
    
    Store at -20°C
    
    SMILES
    
    O=C(NC(C(NC(C(NC(C(NC(CC(O)=O)C(NC1=CC=C([N+]([O-])=O)C=C1)=O)=O)C(C)C)=O)CCC(O)=O)=O)CC(O)=O)OCC2=CC=CC=C2
    
    * For Research Use Only