Ac-VEID-AFC

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    Ac-VEID-AFC is a fluorogenic substrate of caspase-6 (Km=30μM; Kcat/Km=168000 M-1sec-1) and related cysteine proteases. The sequence is based on the lamin A site of cleavage. During apoptosis, caspase-6 cleaves the substrate and release AFC which then can be quantified by fluorescent detection at Ex/Em = 400/505. Ac-IETD-pNA can be used to identify and quantify caspase-6 activity in apoptotic cell lysates and to study events downstream of caspase-6 activation.
    
    1. A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis: N.A. Thornberry, et al.; J. Biol. Chem. 272, 17907 (1997)
    2. Substrate specificities of caspase family proteases: R.V. Talanian, et al.; J. Biol. Chem. 272, 9677 (1997)
    3. The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32: S.M. Srinivasula, et al.; J. Biol. Chem. 271, 27099 (1996)
- Properties
  - Alternative Name
    
    10-(sec-butyl)-7-(2-carboxyethyl)-4-isopropyl-2,5,8,11-tetraoxo-13-((2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl)carbamoyl)-3,6,9,12-tetraazapentadecan-15-oic acid
    
    Molecular Formula
    
    C32H40F3N5O11
    
    Molecular Weight
    
    727.68
    
    Appearance
    
    A solid
    
    Purity
    
    98.00%
    
    Solubility
    
    ≥72.8 mg/mL in DMSO; insoluble in EtOH; insoluble in H2O
    
    Storage
    
    Store at -20°C
    
    SMILES
    
    O=C(O)CCC(NC(C(NC(C)=O)C(C)C)=O)C(NC(C(C)CC)C(NC(CC(O)=O)C(NC1=CC(O2)=C(C=C1)C(C(F)(F)F)=CC2=O)=O)=O)=O
    
    * For Research Use Only

Documents

COA (Certificate Of Analysis)

MSDS (Material Safety Data Sheet)

Ac-VEID-AFC

Background

Documents

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