Ac-IETD-pNA

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    Ac-IETD-pNA is a colorimetric substrate for caspase-8 and other similar cysteine proteases (caspase-6, caspase-10 and granzyme B). It recognizes the amino acid sequence IETD which is based on caspase-8 cleavage site in Caspase-3 precursor. During apoptosis, caspase 8 cleaves the substrate and releases pNA, thus the caspase activity can be calculated by the spectrophotometric detection of pNA at 405nm. Ac-IETD-pNA can be used to identify and quantify caspase-8 activity in apoptotic cell lysates and to study events downstream of caspase-8 activation.
    
    1. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature 376, 37 (1995)
    2. Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik, et al.; Nature 371, 346 (1994)
    3. Interleukin-1 beta converting enzyme: N.A. Thornberry; Methods Enzymol. 244, 615 (1994)
- Properties
  - Alternative Name
    
    (4S,7S,10S,13S)-4-((S)-sec-butyl)-7-(2-carboxyethyl)-10-((R)-1-hydroxyethyl)-13-((4-nitrophenyl)carbamoyl)-2,5,8,11-tetraoxo-3,6,9,12-tetraazapentadecan-15-oic acid
    
    CAS Number
    
    219138-21-3
    
    Molecular Formula
    
    C27H38N6O12
    
    Molecular Weight
    
    638.62
    
    Appearance
    
    A solid
    
    Purity
    
    98.00%
    
    Solubility
    
    ≥63.9 mg/mL in DMSO; ≥14.03 mg/mL in H2O; ≥5.99 mg/mL in EtOH with ultrasonic
    
    Storage
    
    Store at -20°C
    
    SMILES
    
    O=C(O)CC[[email protected]](NC([[email protected]@H](NC(C)=O)[[email protected]@H](C)CC)=O)C(N[[email protected]@H]([[email protected]](O)C)C(N[[email protected]@H](CC(O)=O)C(NC1=CC=C([N+]([O-])=O)C=C1)=O)=O)=O
    
    * For Research Use Only

Documents

COA (Certificate Of Analysis)

MSDS (Material Safety Data Sheet)

Ac-IETD-pNA

Background

Documents

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