Ac-VEID-pNA

- Overview
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    Background
    
    Ac-VEID-pNA is a colorimetric substrate for caspase-6 (Km=30µM; Kcat/Km=168’000 M-1sec-1), and other similar cysteine proteases, which recognize the amino acid sequence VEID. The sequence is based on the lamin A site of cleavage. During apoptosis, Caspase 6 cleaves the substrate and releases pNA, thus the caspase activity can be calculated by the spectrophotometric detection of pNA at 405nm (ε=10,500 M-1cm-1). Ac-VEID-pNA can be used to identify and quantify caspase-6 activity in apoptotic cell lysates and to study events downstream of caspase-6 activation.
    
    1. A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis.: N.A. Thornberry et al.; J. Biol. Chem. 272, 17907 (1997)
    2. Substrate specificities of caspase family proteases.: R.V. Talanian et al.; J. Biol. Chem. 272, 9677 (1997)
    3. The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32.: S.M. Srinivasula et al.; J. Biol. Chem. 271, 27099 (1996)
- Properties
  - Alternative Name
    
    10-(sec-butyl)-7-(2-carboxyethyl)-4-isopropyl-13-((4-nitrophenyl)carbamoyl)-2,5,8,11-tetraoxo-3,6,9,12-tetraazapentadecan-15-oic acid
    
    CAS Number
    
    189684-54-6
    
    Molecular Formula
    
    C28H40N6O11
    
    Molecular Weight
    
    636.65
    
    Appearance
    
    A solid
    
    Purity
    
    98.00%
    
    Solubility
    
    ≥63.7 mg/mL in DMSO; insoluble in EtOH; insoluble in H2O
    
    Storage
    
    Store at -20°C
    
    SMILES
    
    O=C(O)CCC(NC(C(NC(C)=O)C(C)C)=O)C(NC(C(C)CC)C(NC(CC(O)=O)C(NC1=CC=C([N+]([O-])=O)C=C1)=O)=O)=O
    
    * For Research Use Only