Name: Tosufloxacin Tosylate
Price: 208.00 USD

- Overview
  - Tosufloxacin Tosylate is a fluoroquinolone antibacterial agent. It is a small organic compound and racemate, comprising equimolar amounts of (R)- and (S)-tosufloxacin tosylate. It can act as a mimetic of the neural glycan LewisX (LeX), thus it can influence distinct signal transduction pathways involved with diseases of the nervous system.
    
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    Background
    
    Tosufloxacin inhibits bacterial DNA gyrase (topoisomerase).
- Properties
  - CAS Number
    
    115964-29-9
    
    Molecular Formula
    
    C19H15F3N4O3 ∙C7H8O3S
    
    Molecular Weight
    
    576.54
    
    Appearance
    
    White or pale yellow powder
    
    Solubility
    
    Slightly soluble in water
    
    Other Properties
    
    Source: Synthetic
    
    Storage
    
    <30°C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Effective against Gram-positive and Gram-negative bacteria inlcuding anaerobic bacteria, Chlamydia, and Mycoplasma.
    
    Microbiology Applications: Tosufloxacin is commonly used for in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. Representative MIC values include: Pseudomonas aeruginosa 0.1 µg/mL Staphylococcus aureus 0.025 µg/mL
    Eukaryotic Cell Culture Applications: LewisX (LeX) is an abundantly expressed neural glycan. Using an ELISA assay with LeX-specific monoclonal antibodies (L5 and SSEA-1), authors screened a library of small organic molecules. Tosufloxacin was found to be a mimetic compound that can mimic LeX both structurally and functionally, inhibiting antibody binding in a concentration-dependent manner. In nM concentrations, it can influence distinct signal transduction pathways, and enhance neurite outgrowth and promote neuronal survival of cultured mouse cerebellar granule cells. This is a powerful tool for studying the functions of LeX and its effects in acute and chronic nervous system disease models (Theis et al, 2018). Tosufloxacin solution was applied to three corneal cell lines (rabbit corneal epithelium, bovine corneal epithelium, and rabbit corneal epithelium) and one conjunctival cell line (human conjunctival cells) and cell viability was assessed via MTT assay after 48 hours exposure. Cell viability scores were assessed as CVS50, which means the number of cell line with viability ≥50% in the presence of a 10-fold dilution of the antibiotic solution) were higher with Tosufloxacin compared to dibekacin, levofloxacin, norfloxacin, gatifloxacin and moxifloxacin. The toxicity of the compound was dependent on the pharmaceutical components of the solution (Ayaki et al, 2010).