Short human tissue transglutaminase, aa 1-465

- Overview
  - Short rhTG2 is a truncated variant of TG2 lacking both ß-barrel domains. The protein was expressed in E. coli based on the TGM2-allele from I.M.A.G.E.-clone IMAGp958L121020 isolated from neuroblastoma cells of the human brain (Val224-allele, Kanchan et al., Biochem. J. 2013, 455:261–72). It is N-terminally fused to a hexahistidine-tag. Short rhTG2 was purified by ion metal chelating chromatography (IMAC).
    
    Please contact us at for specific academic pricing.
    
    More Details
    
    Zedira
- Properties
  - Name
    
    Tissue-type Transglutaminase, TG2, TGase 2, proteinglutamine-γ-glutamyltransferase
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Formulation
    
    The transglutaminase is lyophilized from 50 mM NaH2PO4, 150 mM NaCl, pH 8 and less than 0.1 mM Imidazole. Sample contains maltodextrin.
    
    Storage
    
    Store at ≤ - 20°C. Store working aliquots at ≤ - 20°C. Avoid repeated freezing and thawing.
    
    Note
    
    INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
    
    Molecular Weight
    
    53.3 kDa
    
    Class
    
    Human Transglutaminase 2
    
    Activity
    
    The Transglutaminase is activated with 10 mM Ca2+.Important note: In order for the protein to be catalytically active, it is necessary to incubate the working solution with a reducing agent, preferably 20-30 mM DTT, for at least 30 min at room temperature, immediately before use.
- Applications
  - Application Description
    
    rhTG2 catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. rhTG2 may also be used for immunoprecipitation.
- Reference
  - Stamnaes et al, PLOS ONE, 2015, 10:e0134922

Datasheet

MSDS

Short human tissue transglutaminase, aa 1-465

Short human tissue transglutaminase, aa 1-465

More Details

We Also Recommend

Human tissue transglutaminase

Human tissue transglutaminase

Human tissue transglutaminase