Seplife® Chelex7350 Affinity, ionic and adsorption resins (800-1000Å, 100-250µm) - Enzyme Carrier

Seplife® Chelex7350 is a highly cross-linked iminodiacetic acrylate carrier that has the appearance of white spherical beads. The particle size distribution is 100-250 micron. The resin is highly hydrophilic and has a porous structure combined with a very high surface area which is ideal for immobilization of enzymes. Iminodiacetic acrylate resins are ideal enzyme carriers for enzymes that are expressed with a His-tag. The iminodiacetic group, upon chelation with a bivalent ion as Fe2+, Zn2+ , Cu2+, Co2+ , Ni2+ , forms very strong affinity binding with the histidine groups of the His tag for the proteins. The great advantage of using affinity immobilization of enzyme is the possibility to perform in a single step the purification and immobilization, thus optimizing all the downstream processing. The resins are mechanically very strong and can be used in either stirred tank or bed reactor.
The high cross-linking of the resin ensures extremely high mechanical stability thus allowing the possibility to reuse the immobilized enzyme for many cycles.

Key features and Benefits
• Easy immobilization through affinity of iminodiacetic group and histidines
• Good physical and chemical stability
• Long lifetime

Physical and Chemical Characteristics
• Matrix: Polyacrylate
• Appearance: While Opaque Spherical beads
• Functional group: Iminodiacetic (Na+)
• Immobilized method: Affinity
• Moisture content (%): 60-70
• Shipping weight (g/ml): 0.70-0.80
• Particle size (μm): 100-250
• Pore size (Å): 800-1000
• Surface area (m2/g): ≧120
• Chemical stability: The resin is insoluble in water, acid, alkali and methanol, ethanol, acetone, toluene, n-heptane, DMSO and other organic solvents.
• Immobilization: pH 4-10

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