Polymyxin B1 Sulfate, EvoPure®

- Overview
  - Polymyxin B1 Sulfate, EvoPure® is one of the polypeptide fractions in Polymyxin B Sulfate. The fatty acid group found in Polymyxin B1 Sulfate is 6-methyloctanoic acid (6-MOA); the same group found in Polymyxin B1-I Sulfate. Polymyxin B1 however, contains a L-leucine residue, rather than the L-isoleucine reside found in Polymyxin B1-I sulfate. Results from in vitro studies have shown marginal differences in MIC data when comparing the fractions.
    
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    Background
    
    Polymyxin B targets and alters the permeability of lipopolysaccharide (LPS) of Gram-negative bacteria leading to cell lysis. Polymyxin B only needs to interact with LPS, it is not required to enter the cell.
- Properties
  - CAS Number
    
    4135-11-09
    
    Molecular Formula
    
    C56H98N16O13 · xH2SO4 (lot specific)
    
    Molecular Weight
    
    1203.47 g/mol (Free base)
    
    Appearance
    
    White powder
    
    Other Properties
    
    Source: Pseudomonas sp.
    Purity Level: >90.0%
    
    Storage
    
    -20°C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Polymyxin B Sulfate targets the outer membrane of Gram-negative bacteria especially Pseudomonas aeruginosa.
    
    Microbiology Applications: Polymyxin B Sulfate is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. Representative MIC values include:
    Pseudomonas aeruginosa 0.25 µg/mL – 1 µg/mL
    
    Plant Biology Applications: Polymyxin was successfully tested to counteract phytopathogenic Gram-negative bacteria growth. Polymyxin reduced growth of different strains of Pseudomonas viridiflava at low concentrations (0.08 µg/ml) whereas Erwinia carotovora growth was inhibited at slightly higher concentrations (0.25 µg/ml) (Selim et al, 2005).