Pig tissue transglutaminase

Pig tissue transglutaminase

Catalog Number:
E001055279ZED
Mfr. No.:
T159
Price:
$774
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      • Overview
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          Background

          sTG2 is based on the NCBI database sequence XM_003359989. It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MHHHHHHAEDLIL….sTG2 is produced in E. coli and purified by ion metal chelating chromatography to more than 95 % purity.

          More Details

      • Properties
        • Name
          Tissue-type Transglutaminase, TG2, TGase 2, proteinglutamine-γ-glutamyltransferase
          Purification
          > 95 % (visually by SDS-PAGE)
          Formulation
          The enzyme is lyophilized from 50 mM NaH2PO4, 150 mM NaCl, pH 8.0. Sample contains maltodextrin.
          Storage
          Store at -20°C in working aliquots. Repeated freezing and thawing is not recommended. Delivery is possible at ambient temperature
          Note
          INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
          Molecular Weight
          78 kDa
          Class
          Animal Transglutaminase 2
          Reconstitution
          Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution, the solution should be stored frozen in working aliquots. Keep cooled on ice for short term storage.
          Activity
          Add 10 mM Ca2+ to activate sTG2.
      • Applications
        • Application Description
          sTG2 catalyzes acyl transfer reactions from glutamin residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. sTG2 may also be used for immunoprecipitation.

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