pAAVdual-Tie1-EGFP

pAAVdual-Tie1-EGFP is used to produce AAV-Tie1-EGFP virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a Tie1 promoter and an EGFP reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-Tie1-EGFP viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The Tie1 promoter is a regulatory sequence that controls the expression of the Tie1 (Tyrosine kinase with immunoglobulin-like and EGF-like domains 1) gene. Tie1 is a receptor tyrosine kinase that plays a critical role in the development and maintenance of the vascular system, particularly in endothelial cells, which line the interior surface of blood vessels. The Tie1 promoter is an endothelial cell-specific regulatory element that drives gene expression in the vascular endothelium. It is a powerful tool in vascular research, gene therapy, and the development of transgenic models for studying blood vessel formation, endothelial function, and related diseases. Its specificity to endothelial cells makes it invaluable for targeting gene expression within the vascular system, particularly in the context of angiogenesis and vascular disorders.

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