pAAVdual-Grm6-EGFP

pAAVdual-Grm6-EGFP is used to produce AAV-Grm6-EGFP virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a Grm6 promoter and an EGFP reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-Grm6-EGFP viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The GRM6 promoter is the regulatory region responsible for controlling the expression of the Glutamate Receptor, Metabotropic 6 (GRM6) gene. This gene encodes a metabotropic glutamate receptor that is primarily expressed in the retina, specifically in the ON bipolar cells. ON bipolar cells play a crucial role in the visual system by processing light signals and transmitting them to the brain. It is an important tool in retinal gene therapy, vision research, and the development of transgenic models to study retinal function and diseases affecting ON bipolar cells. The specificity of the GRM6 promoter to ON bipolar cells makes it invaluable for targeting gene expression in the visual system.

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