pAAVdual-TERT-EGFP

pAAVdual-TERT-EGFP is used to produce AAV-TERT-EGFP virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a TERT promoter and an EGFP reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-TERT-EGFP viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The TERT promoter refers to the regulatory sequence that controls the expression of the telomerase reverse transcriptase (TERT) gene. TERT is the catalytic subunit of the enzyme telomerase, which plays a crucial role in maintaining telomere length and thus in cellular aging and the lifespan of cells. The TERT promoter is particularly significant in cancer research, as telomerase activity is often upregulated in cancer cells, allowing them to proliferate indefinitely. The TERT promoter is also active in stem cells and certain progenitor cells, where telomerase activity is necessary for long-term self-renewal and tissue regeneration. In most normal somatic cells, the TERT promoter is relatively inactive, leading to limited telomerase activity and eventual cell aging and senescence.

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