pAAVdual-ACTA1-EGFP

pAAVdual-ACTA1-EGFP is used to produce AAV-ACTA1-EGFP virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a ACTA1 promoter and an EGFP reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-ACTA1-EGFP viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The ACTA1 promoter is a regulatory DNA sequence that controls the expression of the ACTA1 gene, which encodes alpha-skeletal actin, a key component of the contractile apparatus in skeletal muscle cells. This promoter is highly specific to skeletal muscle tissue, making it a valuable tool in research and gene therapy applications where muscle-specific gene expression is desired.

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