pAAVdual-Nanog-EGFP

pAAVdual-Nanog-EGFP is used to produce AAV-Nanog-EGFP virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a Nanog promoter and an EGFP reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-Nanog-EGFP viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The Nanog promoter is the regulatory sequence that controls the expression of the Nanog gene, a key transcription factor involved in maintaining the pluripotency and self-renewal of embryonic stem cells. Nanog is crucial for keeping stem cells in an undifferentiated state and is highly expressed in early embryonic development. The Nanog promoter is a pluripotency-specific regulatory element that drives gene expression in embryonic stem cells and induced pluripotent stem cells. It is a vital tool in stem cell research, the generation of iPSCs, and the development of transgenic models to study pluripotency, early embryonic development, and cell differentiation. Its specificity to pluripotent stem cells makes it invaluable for targeting gene expression in studies focused on stem cell biology and regenerative medicine.

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