pAAVdual-Iba1-EGFP

pAAVdual-Iba1-EGFP is used to produce AAV-Iba1-EGFP virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a Iba1 promoter and an EGFP reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-Iba1-EGFP viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The Iba1 promoter refers to the regulatory sequence that controls the expression of the Ionized calcium-binding adaptor molecule 1 (Iba1) gene. Iba1 is a protein highly expressed in microglia, the resident immune cells of the central nervous system (CNS), as well as in macrophages. The Iba1 promoter is a microglia- and macrophage-specific regulatory element that drives gene expression in these immune cells, particularly within the CNS. It is an essential tool in neuroscience research, gene therapy for CNS diseases, and the development of transgenic models to study microglia and macrophage function. Its specificity to microglia makes it invaluable for targeting gene expression in studies focused on neuroinflammation, neurodegeneration, and CNS immune responses.

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