pAAVdual-EnSM22a-EGFP

pAAVdual-EnSM22a-EGFP is used to produce AAV-EnSM22a-EGFP virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a EnSM22a promoter and an EGFP reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-EnSM22a-EGFP viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The EnSM22a promoter is chimeric vascular smooth muscle-specific enhancer/promoter based on the SM22α (Smooth Muscle 22 alpha) promoter, a regulatory element that controls the expression of the SM22α gene. SM22α, also known as transgelin, is a protein predominantly expressed in smooth muscle cells and plays a key role in smooth muscle cell differentiation and function. This engineered promoter combines elements from natural smooth muscle-specific regulatory sequences, optimizing them for enhanced specificity and activity in vascular smooth muscle cells (VSMCs).

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