pAAVdual-CD68-EGFP

pAAVdual-CD68-EGFP

Catalog Number:
VP01554532AAV
Mfr. No.:
SL029001
Price:
$1,499
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      • Overview
        • pAAVdual-CD68-EGFP is used to produce AAV-CD68-EGFP virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a CD68 promoter and an EGFP reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-CD68-EGFP viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
          The CD68 promoter is a regulatory sequence that controls the expression of the CD68 gene, which encodes a glycoprotein primarily expressed on the surface of macrophages, monocytes, and other cells of the mononuclear phagocyte system. CD68 is commonly used as a marker for these immune cells, particularly in studies involving inflammation, immune responses, and diseases related to the immune system.

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      • Properties
        • Other Properties
          AAV Cassette: AAV-CD68-EGFP
          AAV Genome Size: 2291 bp
          Plasmid Size: 10724 bp
          Plasmid Backbone: pAAVdual
          AAV Genome Type: ssAAV
          ITRs: L-ITR (119 bp) + R-ITR (130 bp)
          Promoter: CD68
          Transgene: EGFP
          Bacterial Resistance(s): Kana
          Storage
          -20°C for long term (> 1 year)
          Shipping
          Dry ice.
      • Applications
        • Application Description
          pAAVdual plasmids are used to produce regular single strand AAV (ssAAV) viruses using novel AAVdual System. In this series plasmids, the AAV genomes are cloned into our novel Ad helper plasmid, mini-pHelper. AAV viruses can be generated by co-transfection of pAAVdual plasmids with the regular AAV helper (pRCap) plasmids carrying different AAV serotypes.

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