pAAVdual-Col2a1-EGFP

pAAVdual-Col2a1-EGFP is used to produce AAV-Col2a1-EGFP virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a Col2a1 promoter and an EGFP reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-Col2a1-EGFP viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The Col2a1 promoter refers to the regulatory sequence that controls the expression of the Collagen Type II Alpha 1 (Col2a1) gene. The Col2a1 gene encodes type II collagen, which is a major component of cartilage, making this promoter particularly important in the context of chondrocyte biology and cartilage-related research. The Col2a1 promoter is highly specific to chondrocytes, the cells responsible for producing and maintaining the cartilaginous matrix in joints and other cartilaginous tissues. This specificity makes it a crucial tool for targeting gene expression in cartilage.

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